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617 Exposure of astrocytes to -amyloid down-regulates expression of glutamine synthetase, 618 and in animal models, glutamine synthetase expression is reduced in astrocytes surrounding -amyloid plaques

The membranes were blocked with 5% BSA and incubated with primary antibodies at 4 C for 12 h, followed by secondary antibodies (HRP-conjugated Goat Anti-Mouse IgG, Proteintech, SA00001-1, 1:20000) for 1 h at room temperature

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